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FAQ – ALM

Leica TCS SP8 Confocal Microscopes

  • About FAQ
  • Error: Socket controller VI timeout
  • Function Keys on smartmove and microscope
  • Guidelines for booking the SP8 Confocal Microscopes
  • How do I start-up the Leica TCS SP8 microscope
  • How to keep the objectives clean when working with oil-immersion
  • LAS-X Stage Applications (temporarily only on the Widefield DM6 and Confocal SP8-DLS)
  • Leica Super Z-Galvo stage
  • ML-II rules
  • Tips and tricks confocal stainings
  • Use cover glass with thickness No. 1.5H (170 nm High performance)
  • Using the SP8-DLS as a Standard Confocal Microsope
  • Which Immersion Oil to use and how to use it

 

Leica SR-GSD Super-resolution Microscope

  • About FAQ
  • Starting the Leica SR-GSD Microscope
  • Use cover glass with thickness No. 1.5H (170 nm High performance)
  • Which Immersion Oil to use and how to use it

 

Live Cell Fluorescence Microscopes

  • About FAQ
  • Which Immersion Oil to use and how to use it

 

Wide Field Fluorescence Microscopes

  • About FAQ
  • Which Immersion Oil to use and how to use it

 

Advanced Light Microscopy

Microscope Techniques

  • Confocal Microscopy
  • Thunder Microscope
  • Live-Cell Microscopy
  • High Content Screening
  • Wide-Field Microscopy
  • Lightsheet Microscopy
  • Super-Resolution Microscopy

Special Techniques

  • X-Ray micro-irradiation

Tools

  • Fluorphores Database
  • Zoom Factor/Z-Stack Step Size calculation

Software

  • Leica LAS-X
  • Huygens Deconvolution
  • SymPhoTime 64
  • CI Convert
  • CI Micro

Information

  • Frequently Asked Questions
  • Access to Data and Image Files
  • Nyquist Rate Calculator
  • FRET with FLIM
  • Sample Preparation
  • Documentation Leica TCS SP8
  • Documentation Leica SR GSD
  • Documentation Leica SP8 DLS
Cellular Imaging - Advanced Light Microscopy, Flow Cytometry and Electron Microscopy Center Amsterdam