The Huygens software package runs on a dedicated server. Access to this server is restricted to the eight Cellular Imaging Workstations at our facility. You can use you’re AMC account to login after you get the permission from one of the confocal operators, Ron Hoebe,
Some advantages of the Huygens deconvolution:
- Multi channel images are processed sequentially, allowing you to select the best result of each channel.
- Time series deconvolution of 3D and 2D images (multi)-channel images is extremely easy by selecting time series as one file.
- The intelligent 4D-channel cropping tool allows you to trim images along four dimensions and delete uninteresting channels to reduce the load on your computer’s processing power.
- The Batch Processor allows the scheduling of massive amounts of data with pre-made parameter templates. You can continue with your other research work when Huygens delivers the data you need, quickly and reliably.
The various Visualization Tools in Huygens Essential and Professional help you to analyze your image in detail. With the Twin Slicer and the Gallery Tool you can easily view your multi-dimensional data, and compare your results with the original image side-by-side. A MIP Renderer enables you to obtain a spatial projection of your data from any given point of view. TheOrtho-slicer gives you information of a specific point or area in all three dimensions. The SFP Renderer is also available for visualizing your object from different angles and gives you physically realistic detailed views of your 3D data over time, while the Surface Renderer and the Movie Maker make the visualization of dynamic objects in your data very easy. All visualization tools can be used without needing an expensive graphics card, because our tools do not use conventional techniques of polygonal rendering.
The Twin Slicer
The Twin Slicer allows you to synchronize views of two images, measure distances, plot line profiles, etc. In Basic Mode, image comparison is intuitive and easy, while the Advanced Mode gives the user the freedom to rotate the cutting plane to any arbitrary orientation, link (synchronize) or unlink viewing parameters between the two images, and more.
The Twin Slicer (click to open larger view).
The MIP Renderer
The maximum intensity projection (MIP) renderer projects the voxels with maximum intensity that fall in the way of parallel rays traced from the viewpoint to the plane of projection. This allows you to obtain a direct spatial projection of your 3D microscopy data from the viewpoint you wish.
The MIP Renderer (click to open larger view).
The SFP Renderer
The Volume Renderer or SFP Renderer is based on taking the 3D microscopy image as a distribution of fluorescent material, simulating what happens if the material is excited and how the subsequently emitted light travels to the observer. The computational work is done by the Simulated Fluorescence Process algorithm. The unique properties of this algorithm enable it to create depth cue rich images from unprocessed data.
Because the SFP algorithm does not rely on boundaries or sharp gradients, it is eminently suited to render 3D microscopic data sets. The SFP algorithm is based on ray tracing it does not require a special graphic card.
The Huygens SFP Renderer (click to open larger view).
The Ortho-Slicer is designed to show the same point in 3D space from 3 orthogonal directions. If you move one of the slices, the others will follow to make sure that the center of each of the slices intersects in the same point in space. This behaviour makes the Ortho Slicer a useful tool to study small objects in 3D.
The Surface Renderer
The Surface Renderer enables you to represent your microscopy data in a convenient way to clearly see separated volumes. Not only is it capable of iso-surface rendering; but also of showing MIP projections together with the surfaces to be used as a reference to the original microscopic voxel data. Because the Surface Renderer is based on rendering continuous surfaces with fast ray tracing algorithms, there is no need for a special graphic card. The fast ray tracers can utilize 64 bit multiprocessor systems, and are therefore able to render very large microscopic volume data to high resolution output images.
The Surface Renderer (click to open larger view).
The Colocalization Analyzer
With the Colocalization Analyzer you can obtain information about the amount of spatial overlap between different data channels, in 3D stacks or 3D time series. As this overlapping can be defined in many ways, Huygens gives you the colocalization coefficients most commonly used in the scientific literature: Pearson, Overlap, and Manders M and K.
The Colocalization Analyzer (click to open larger view).
The Object Analyzer
The Object Analyzer is a great tool to label and analyze 3D and 4D single and multi-channel single objects and their statistics. With the 3D region of interest (ROI) selector tool you can limit the analysis to a certain volume only, but also crop your original data precisely like you want it for further analysis. Next to analyzing single objects or groups you can also analyze the whole dataset in all its aspects by clicking on one single button. This tool provides information about objects in different channels and time points: it reports physical properties, how objects relate spatially to each other or to reference objects, and how they overlap.
The Object Analyzer (click to open larger view).
The Object Stabilizer
The Object Stabilizer can measure and correct for cell motion, thermal drift, shaking, and other types of movement (x-y-z translation and axial rotation). Both the measurement and subsequent stabilization are done in 3D and at sub-pixel level. The Stabilizer not only stabilizes 2D or 3D time series, but it also allows the alignment of slices within a 3D stack.
Images show the sum of four time frames (300 s. lapse) of an apoptotic cell stained with fluorescent H2B nuclear protein. Courtesy of M. Varecha and M. Kozubek, Masaryk University, Czech Republic.
The Object Tracker
The Object Tracker is equipped with a wizard for optimized object detection and fast track filtering. The integrated Track Analyzer analyzes object position, speed and flow, and enables the user to visualize and export results in histograms and plots, and as CSV files.
The Track Analyzer (click to open larger view).