Live-Cell Microscopy

User fee: €10/hr (max €60/hr)

The basis of this system is a Leica DMi8 inverted fluorescence, DIC and phase contrast microscope. The microscope is enclosed in a cabinet and kept at 37 C. Cells can be kept alive for 3-10 days. A highly light sensitive CCD camera is used to obtain fluorescence, DIC and phase contrast images in time. It is possible to obtain stacks of fluorescence images. Images can be acquired at a rate of about 20 per second.
A motorized microscope table allows monitoring several areas of a culture flask in one experiment.

Full specifications..

The basis of this system is a Leica IRBE inverted fluorescence, phase contrast microscope. The larger part of this microscope is enclosed in a cabinet and kept at 37 C. In our experience cells can be kept alive for 3-10 days in this setting. A highly light sensitive CCD camera is used to obtain fluorescence and phase contrast images in time. It is also possible to obtain stacks of fluorescence images. Images can be acquired at a rate of about 4 per second.
A motorized microscope table allows monitoring several areas of a culture flask in one experiment.

Full specifications..

Data can be visualized and processed on dedicated workstations.

  • Timelapse Analyse
  • Leica LAS-X
    • 2D and 3D Visualisation and animations
    • 2D and 3D Measurements and analyzing
  • Matlab (on the LCAM-Remote server)
    • Image processing and quantification
  • Huygens (on the LCAM-Remote server)
    • Image deconvolution to increase resolution and contrast
    • Image processing
    • Visualization