Live-Cell Microscopy

User fee: €10/hr (max €60/hr)

IncuCyte S3

The IncuCyte® S3 Live-Cell Analysis System is a real-time quantitative live-cell imaging and analysis platform that enables visualization and quantification of cell behavior over time by automatically gathering and analyzing images around the clock within a standard laboratory incubator. This allows researchers to make time-lapsed, kinetic measurements from living cells over days and weeks thus providing insight into active biological processes in real time.

Real-time live-cell analysis — Set up & walk away, automatically acquire images for hours, days, or weeks
Preserve cells — Non-perturbing reagent formulations, minimal sample input requirements
Superior data — Automatically acquire presentation- and publication-ready images and movies
Flexibility — Supports multiple users, vessels and applications simultaneously

Leica DMi8

The basis of this system is a Leica DMi8 inverted fluorescence, DIC and phase contrast microscope. The microscope is enclosed in a cabinet and kept at 37 C. Cells can be kept alive for 3-10 days. A highly light sensitive CCD camera is used to obtain fluorescence, DIC and phase contrast images in time. It is possible to obtain stacks of fluorescence images. Images can be acquired at a rate of about 20 per second.
A motorized microscope table allows monitoring several areas of a culture flask in one experiment.

Full specifications..

Leica IRBE

The basis of this system is a Leica IRBE inverted fluorescence, phase contrast microscope. The larger part of this microscope is enclosed in a cabinet and kept at 37 C. In our experience cells can be kept alive for 3-10 days in this setting. A highly light sensitive CCD camera is used to obtain fluorescence and phase contrast images in time. It is also possible to obtain stacks of fluorescence images. Images can be acquired at a rate of about 4 per second.
A motorized microscope table allows monitoring several areas of a culture flask in one experiment.

Full specifications..

Data analysis

Data can be visualized and processed on dedicated workstations.

  • Timelapse Analyse
  • Leica LAS-X
    • 2D and 3D Visualisation and animations
    • 2D and 3D Measurements and analyzing
  • Matlab (on the LCAM-Remote server)
    • Image processing and quantification
  • Huygens (on the LCAM-Remote server)
    • Image deconvolution to increase resolution and contrast
    • Image processing
    • Visualization