Confocal Microscopy

User fee: €10/hr

The Leica TCS SP8 X is mounted on a Leica DMI6000 inverted microscope.
Excitation is possible with UV (405 nm) and VIS (White Light Laser 470-670 nm). Simultaneous detection of 4 separated fluorophores is possible with an additional option for transmitted light. Software includes FRAP and FRET wizards.

Start-up guide  |  LAS-X  | Full specifications..

The Leica TCS SP8 SMD is mounted on a Leica DMI6000 inverted microscope enclosed in incubator at 37° for live-cell experiments . Excitation is possible with UV (405 nm) and VIS (440, 458 and White Light Laser 470-670 nm). Simultaneous detection of 5 separated fluorophores is possible with an additional option for transmitted light. This microscope is equipped an advanced standard and resonant scanner with FALCON Single Molecule Detection (SMD) to perform Fluorescence Life-Time Imaging Microscopy (FLIM). Software includes FRAP and FRET(FLIM) wizards.

Start-up guide |  LAS-X  |  FALCONAdvanced (Resonant) Scanner  | Full specifications..

The Leica TCS SP8 X DLS is mounted on a Leica DMi8 inverted microscope. Excitation is possible with UV (405 nm) and VIS (White Light Laser 470-670 nm). Simultaneous detection of 4 separated fluorophores is possible with an additional option for transmitted light. Software includes FRAP and FRET wizards.

On top of the TCS SP8 X is the DLS lightsheet microscope. The excitation lasers of the SP8 Confocal are used for creating the lightsheet. Detection is done by a fast sCMOS camera.

Start-up guide | Lightsheet  |  LAS-X  | Full specifications..

Data can be visualized and processed on dedicated workstations.

  • Leica LAS-X
    • 2D and 3D Visualisation and animations
    • 2D and 3D Measurements and analyzing
  • Matlab (on the LCAM-Remote server)
    • Image processing and quantification
  • Huygens (on the LCAM-Remote server)
    • Image deconvolution to increase resolution and contrast
    • Image processing
    • Visualization
  • SymPhoTime 64
    • FRET with FLIM analysis