SP8 LAS-X Dye Assistant

Crosstalk can emerge during multi-channel acquisitions on specimens with several fluorescent dyes. The emission spectra of the dyes can overlap as a result, causing mixing of the emissions in this range.

The Dye Assistant offers suggestions on system configuration based on the spectral characteristics of the fluorescent agents being used. The user can then select the appropriate option for his or her application. Due to the specific characteristics and the relative expression level of the specimen being used, the user may have to carry out subsequent optimization.

	The fluorescent dyes used for the specimen are selected in this area. Clicking on the (…) button opens a selection list with the default standard fluorescent dyes. If the system is equipped with one or more HyD detectors, the individual fluorescent dyes can be assigned to a specific detector type. However, this may cause individual configurations to be unavailable. Example: If two fluorescent dyes in use are assigned to a HyD detector but the system is only equipped with one HyD detector, this results in a configuration in sequential mode (stack-by-stack or frame-by-frame).
	The + button lets you add fluorescent dyes. The – button lets you remove the highlighted fluorescent dye.
	The different configuration options for acquisition of the specimen being used are suggested here and the overlap is shown graphically. Yield: Intensity yield of the individual fluorescent dyes Crosstalk: Intensity of the crosstalk in other channels
	Opens an additional dialog for additional settings for the laser wavelength and the detection range.
	The Apply button applies the corresponding configuration for image acquisition. The following settings are made for image acquisition when clicking Apply: Selection of the laser lines Selection of the detectors Setting for the detection range Assignment of the fluorescent dyes to the respective detectors Assignment of the appropriate color look-up table (LUT) for the respective fluorescent dye All other settings for image acquisition are made as usual.

The Edit (4) function opens an additional dialog.

	The vertical lines within the emission curves represent excitation laser lines. The short, gray lines above the emission curves represent the laser lines in the instrument. The laser lines can be moved using the arrows and snap in place at the short gray lines. The excitation wavelength of the white light laser can be configured at any time.
	In this case, the wavelength range the detector records can be configured by moving the colored bar.
	Reset: Any settings that have been made are reset to the calculated settings. Apply: Any settings that have been made are applied to image acquisition directly.
	OK: Any settings that have been made are applied in the main window of the Dye Assistant. Cancel: Closes the dialog.