The flow facility is equipped with multiple different sorters, each with its own possibilities. Machines are available 24/7 on an autonomous basis following a theoretical and practical introduction provided by the core staff. Or sorts can be done by one of the operators.
Sony SH800
- 4 Colinear lasers (405nm, 488nm, 561nm, 638nm)
- Up to 6 colours (excluding FSC/SSC)
- Can sort 2 populations simultaneously
- Can sort into 15 ml tubes, 5 ml tubes, eppendorf tubes and plates
- Single cell sorting into 96 and 384 plates
- Index sorting
- Different nozzle sizes available:
- 70 µm nozzle/40 psi/10000 events/sec
- 100 µm nozzle/20 psi/5000 events/sec
- 130 µm nozzle/9 psi/2000 events/sec
BD FacsAria II SORP
- 4 Spatially separated lasers (405nm, 488nm, 561nm, 640nm)
- Up to 13 colours (excluding FSC/SSC)
- Can sort 4 populations simultaneously
- Can sort into 15 ml tubes, 5 ml tubes, eppendorf tubes, plates and on slides
- Single cell sorting into 96 and 384 plates
- Index sorting
- Different nozzle sizes available:
- 85 µm nozzle/45 psi/10000 events/sec
- 100 µm nozzle/20 psi/6000 events/sec
BD Symphony S6
- 4 Spatially separated lasers (405nm, 488nm, 561nm, 640nm)
- Up to 16 colours (excluding FSC/SSC)
- Can sort 6 populations simultaneously
- Can sort into 15 ml tubes, 5 ml tubes, eppendorf tubes, plates and on slides
- Single cell sorting into 96 and 384 plates
- Index sorting
- Different nozzle sizes available:
- 85 µm nozzle/45 psi/10000 events/sec
- 100 µm nozzle/20 psi/6000 events/sec
BD Influx
- 4 Spatially separated lasers (405nm, 488nm, 561nm, 640nm)
- Up to 14 colours (excluding FSC/SSC)
- Can sort 6 populations simultaneously
- Can sort into 15 ml tubes, 5 ml tubes, eppendorf tubes, plates and on slides
- Single cell sorting into 96 and 384 plates
- Index sorting
- Different nozzle sizes available:
- 86 µm nozzle/33 psi/20000 events/sec
- 140 µm nozzle/5 psi/3000 events/sec
- 200 µm nozzle/3 psi/2000 events/sec