The flow facility is equipped with multiple different analysers, each with its own possibilities. All machines are available 24/7 on an autonomous basis following a theoretical and practical introduction provided by the core staff.
BD LSR Fortessa
- 5 Spatially separated lasers (365nm, 405nm, 488nm, 561nm, 640nm)
- Up to 18 colours (excluding FSC/SSC)
- High Throughput Sampler (HTS) attached to analyse directly from 96 well plates
- Equipped for Ca+ flux experiments
BD FacsCantoII
- 3 Spatially separated lasers (405nm, 488nm, 633nm)
- Up to 8 colours (excluding FSC/SSC)
- High Throughput Sampler (HTS) attached to analyse directly from 96 well plates
BD Symphony A1
- 4 Spatially separated lasers (405nm, 488nm, 561nm, 637nm)
- Up to 16 colours (excluding FSC/SSC)
- High Throughput Sampler (HTS) attached to analyse directly from 96 well plates
Sony SP6800 Spectral
- 3 lasers (488nm and 405nm/638nm)
- Full spectrum Cytometer, which allows for unconventional fluorophore combinations
- Autofluorescence reduction
- No compensation required
Sony ID7000 Spectral
- 6 lasers (320nm, 355nm, 4o5nm, 488nm, 561nm and 637nm)
- 182 PMT fluorescent detectors
- Full spectrum Cytometer, which allows for unlimited fluorophore combinations
- Autofluorescence reduction
- Cooled autosampler for 24 tubes and all plate formats
Amnis Imagestream X MkII
- 4 lasers (488nm/ 561nm and 405nm/642nm)
- Up to 10 colours
- Gives quantitative data along with images
- Camera operates with 20X/40X/60X magnification with a pixel size of 0,1/0,25/1 µm2
- Equipped with a plateloader to analyse directly from 96 wells plates
Data Workstations data can be processed on a dedicated workstations with FlowJo Software. All flow cytometry workstations (A-E) are also equiped with High Dimensional Single Cell Data analysis Software.